Protocols

Three Dimensional Growth of MCF10A in Matrigel	10A_Morphogenesis_EL.pdf
DQ Collagen Assay in Matrigel	DQ_Matrigel.pdf
Seeding of MCF10A for 3D morphogenesis	Seeding_of_MCF10A_for_3D.doc.pdf
Generation of Collagen: Matrigel mixed Gel	Generation_of_Collagen.doc_0.pdf
LDH Assay (for quantification of 3D lysates)	LDH.doc.pdf
Ethidium Bromide staining of MCF 10A acini	EtBr_staining_EL.pdf
Collagen/Matrigel mixed cultures for MCF-10A cells	Collagen_EHS_morphogenesis.pdf
Care and Passage of MCF-10A Cells In Monolayer Culture	Care_Passage_MCF_Matrigel.doc.pdf
Acrobat Immunofluorescence of Mammary Epithelial Acini	3D_IF_Protocol_EL.pdf
Freezing Cells	Freezing_Cells.doc.pdf
Embedding Protocol for MCF-10A Frozen Sections	EmbeddingProtocoMCF_frozen.pdf
Overlay Three-Dimensional Culture of MCF-10A Cells on MatrigelTM	3DimensionalCulture_of_MCF-10A.doc.pdf
Staining Protocol for MCF-10A Frozen Sections	StainingProtocol_MCF_Frozen.pdf
Harvesting Protein Lysates from 3-D Acinar Cultures (for quantification of non-phosphorylated, intracellular, stable proteins)	HarvestingProteinLysates_Stable.doc.pdf
Harvesting Protein Lysates from 3-D Acinar Cultures (for quantification of phospho-proteins, extracellular proteins, or quickly degraded proteins)	Harvesting_Protein_Lysates_QuicklyDegraded.pdf
Harvesting RNA from 3-D Acinar Cultures	HarvestingRNA_3D_AcinarCultures.doc.pdf
Acrobat Ethidium Bromide Staining For Analysis of Cell Death during 3-D Culture	EthidiumBromideStainingAnalysisCellDeath.doc.pdf
Indirect Immunofluorescent Staining of MCF-10A Acini Cultured In Matrigel (Simplified)	ImmunofluorescentStainingMCF-10A_Acini_Simplified.doc.pdf
Indirect Immunofluorescent Staining of MCF-10A Acini Cultured In Matrigel (Detailed)	ImmunofluorescentStainingMCF-10A_Acini_Detailed.doc.pdf
Immunofluorescence Wash Buffer Recipe	BUFFER_RECIPES.doc.pdf
MCF-10A Media Recipes	MediaRecipesForMCF10ACells.pdf
Useful Antibodies And Fluorescent Reagents For The Analysis of MCF-10A Mammary Acini	UsefulAntibodiesAndFlourescentReagents.pdf